This dataset was produced through the joint collection of Statistics Canada's Canadian Wastewater Survey (CWS) with the Public Health Agency of Canada. The CWS measures levels of SARS-CoV-2 in the wastewater of five Canadian municipalities: Vancouver, Edmonton, Toronto, Montreal, and Halifax. The dataset includes measurements by RT-qPCR of the concentration of SARS-CoV-2 and Pepper Mild Mottle Virus (PMMV) in wastewater from 2021/04/01 to 2021/12/15 reported in the Public Health Environmental Surveillance Open Data Model v1.1.