Biomonitoring provides a measure of internal doses of environmental chemicals or agents and allows for a more accurate measure of human health risk from these exposures. In these Alberta Biomonitoring Program datasets, information was collected as part of 3 province-wide studies investigating the serum levels of environmental chemicals (both natural and synthetic) in pregnant women in northern, central and southern Alberta (Phase 1), in children in southern Alberta (Phase 2), and maternal and umbilical cord blood samples from 7 Alberta cities (Phase 3). The goals of the program are to create a benchmark against which to track future exposures, provide a starting point for assessing health risks, indicate possible exposure sources and prioritize research efforts. Chemicals targeted for monitoring were selected using expert guidance and review of similar studies. For Phases 1 and 2, results for each chemical are presented in a single column, as either whole serum or lipid-adjusted concentrations. Each row provides the mean concentration and standard error of measurement for the replicate pools in the Age+Region group. Supplementary rows include the Limit of Detection/Limit of Quantification (LOD/LOQ) and Comments (for report status; results are only reported if ≥25% of pooled samples had detectable concentrations of a chemical). For Phase 3, results for each chemical are presented in up to 8 columns (age-weighted and age-and-geography-weighted means in whole serum and lipid-adjusted serum, and their 95% confidence intervals (CIs)). Each row provides the mean concentration and 95% CI for the replicate pools in the Age+Region group. Supplementary rows include the LOD/LOQ, Report Status (Yes: ≥25% of pooled samples had detectable concentrations; No: <25% had detectable concentrations) and Comments. For more information, please consult the Phase 1 and 2 reports (https://open.alberta.ca/publications/9780778566953; https://open.alberta.ca/publications/9780778582786).