Three marine spatial planning areas are delineated in Eastern Canada to define the spatial extents of marine spatial plans being led by Fisheries and Oceans Canada (DFO): the Estuary and Gulf of St. Lawrence (EGSL), the Newfoundland and Labrador (NL) Shelves, and the Scotian Shelf and Bay of Fundy. The EGSL planning area includes the St. Lawrence River estuary from northeast of Île d’Orléans, Quebec, the Saguenay River estuary, and the entire Gulf of St. Lawrence as far north as the Strait of Belle Isle (NAFO Divisions 4RST). The NL Shelves planning area includes areas off southern, eastern and northern Newfoundland, part of the Churchill River and Lake Melville, as well as off the Labrador coast to the extent of the exclusive economic zone (EEZ) (NAFO Divisions 2GHJ and 3KLNOP). The Scotian Shelf and Bay of Fundy planning area includes DFO Maritimes’ administrative region off the Atlantic coast of Nova Scotia to the extent of the EEZ, the Bay of Fundy and the Canadian portion of the Gulf of Maine (NAFO Divisions 4VWX, 5Ze, and the Canadian portion of 5Y). The French EEZ for St. Pierre et Miquelon is excluded from the three planning areas. These planning areas are derived from Federal Marine Bioregions (https://open.canada.ca/data/en/dataset/23eb8b56-dac8-4efc-be7c-b8fa11ba62e9) that were developed by a Canadian Science Advisory Secretariat process using ecosystem-based management principles to define 13 ecological bioregions that have informed but not directed DFO implementation of marine spatial planning.