In 2018, Fisheries and Oceans Canada initiated the Multidisciplinary Arctic Program (MAP) – Last Ice, the first ecosystem study of the poorly characterized region of Tuvaijuittuq, where multiyear ice still resides in the Arctic Ocean. The program MAP-Last Ice takes a coordinated approach to integrate the physical, biochemical, and ecological components of the sea ice-ocean connected ecosystem and its response to climate and ocean forcings. This program provides baseline ecological knowledge for Tuvaijuittuq and, in particular, for its unique multiyear ice ecosystem. The database provides baseline data on fatty acid composition and stable isotopes signatures of sea ice communities in multi- and first-year ice in Tuvaijuittuq. The data were collected during the 2018 spring field campaign of the MAP-Last Ice Program, offshore of Canadian Forces Station (CFS) Alert, in the Lincoln Sea.