The Brier Island/Digby Neck area has been identified as an Ecologically and Biologically Significant Area (EBSA) by Fisheries and Oceans Canada and is one of four marine areas within the Bay of Fundy recognised by Parks Canada as of national significance for marine conservation planning. The area is representative of important outer Bay of Fundy features with significant marine mammal, bird, and benthic diversity including potentially important aggregations of sensitive benthic species such as horse mussel and sponge. Much of the information used for this recognition is now over 40 years old and should be re-validated using standardised georeferenced survey methods. As a first phase, a diver-based survey of the sublittoral habitats and associated species was conducted in August and September of 2017 for the Brier Island area. This report summarises the major sublittoral habitat types, species assemblages, and oceanographic conditions observed at 20 locations including Northwest and Southwest Ledges, Gull Rock, Peter’s Island, and Grand Passage. A total of 962 records were made of 178 taxa, consisting of 43 algae and 135 animals. Comparison with historical records largely confirmed the continued presence of unique habitats and species assemblages for which this area was initially recognised as an EBSA. Differences in species richness observed for cryptic and less known taxonomic groups such as sponges and bryozoans were attributable to changes in survey methods and knowledge. Based on these findings, additional surveys of inshore and offshore Brier Island using more quantitative methods developed for other Bay of Fundy EBSAs would further support regional MPA network planning and provide relative scales of species diversity and habitat coverage for this area.